the effect of nanochitosans particles on candida biofilm formation

background and purpose: in people wearing dentures, the growth of various candida species under the prosthesis leads to the formation of biofilm, which can play the role of a reservoir for candida and other kinds of microbes. since nano-chitosan particles can cause lasting antimicrobial activity, a more recent approach that utilizes acrylic resins with nano-chitosan particles is proposed. therefore, we aimed to study the inhibitory effect of nano-chitosan particles on the biofilm formation of candida species in acrylic resins. materials and methods: in this analytical in-vitro study, acrylic resins with nano-chitosan particles with concentrations of 0, 1%, 5%, and 10% were put adjacent to the suspension of candida cells isolated from the individuals’ mouth and biofilm formation on resins was measured and compared. finally, the data were analyzed using kruskal-wallis and chi-square tests. results:the observed differences between unmodified acrylic resin (control) and acrylic resin with nano-chitosan particles in terms of biofilm formation were significant (p<0.05), but no significant difference was found in the formation of biofilm species on resins. conclusion: biofilm formation of candida species depends on acrylic resin type, in a way that by adding nano-chitosan particles to acrylic resins, biofilm formation of candida species was significantly reduced. to decrease the organization of biofilm and denture stomatitis, the use of acrylics with nano-chitosan particles in producing dentures is recommended.